Granzyme B Polyclonal antibody, PBS Only

Granzyme B Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 31521-1-PBS

Synonyms

GZMB, CSPB, CSP B, CGL1, CGL 1

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Validation Data Gallery

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  WB analysis using 31521-1-AP (same clone as 31521-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31521-1-AP (GZMB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31521-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 31521-1-AP (same clone as 31521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 31521-1-AP (Granzyme B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31521-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 31521-1-AP (same clone as 31521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 31521-1-AP (Granzyme B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31521-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 31521-1-AP (same clone as 31521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31521-1-AP (Granzyme B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 31521-1-AP (same clone as 31521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31521-1-AP (Granzyme B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31521-1-PBS in a different storage buffer formulation.

• 

  FC experiment of NK-92 using 31521-1-AP (same clone as 31521-1-PBS)

  1x10^6 NK-92 cells were intracellularly stained with 0.25 ug Granzyme B Polyclonal antibody (31521-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 31521-1-PBS in a different storage buffer formulation.

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Product Information

31521-1-PBS targets Granzyme B in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• Calculated Molecular Weight: 247 aa, 28 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC030195
• Gene Symbol: Granzyme B
• Gene ID (NCBI): 3002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.