Granzyme A Recombinant antibody

• 

  WB analysis of NK-92 using 84583-4-RR

  NK-92 cells were subjected to SDS PAGE followed by western blot with 84583-4-RR (GZMA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Granzyme A Recombinant Antibody for WB, ELISA

Cat No. 84583-4-RR

Clone No.241979E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

GrA, GZMA, Fragmentin 1, EC:3.4.21.78, Cytotoxic T-lymphocyte proteinase 1

---

Tested Applications

• Positive WB detected in: NK-92 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84583-4-RR targets Granzyme A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Granzyme A fusion protein Ag1818
• Full Name: granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC015739
• Gene Symbol: Granzyme A
• Gene ID (NCBI): 3001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P12544
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Granzyme A(GrA, a tryptase) is necessary for target cell lysis in cell-mediated immune responses. It activates mitochondrial outer membrane permeabilization- and caspase-independent cell death pathways by cleaving the mitochondrial complex I component NDUFS3after lys56. Among serine proteases GrA has an unique quaternary structure consisting of a disulphide-linked homodimer of 60kDa linked via Cys93.(PMID: 12819769) This protein has 2 isoforms produced by alternative promoter usage with the MW of 29 and 27 kDa.