Mouse Gm-csf Recombinant antibody, PBS Only (Detector)

Gm-csf Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230286A10

Cat no : 82861-8-PBS

Synonyms

Csf2, Gm CSf, Csfgm, CSF 2, Colony Stimulating Factor 2

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00306-3

  Sandwich ELISA standard curve of MP00306-3, GM-CSF Recombinant Matched Antibody Pair - PBS only. 82861-10-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0512.82861-8-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 pg/mL.

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  Sample test of MP00306-3

  Spleen and lung from one mouse were chopped into 1-2 mm pieces and seeded into approximately 30 mL of media containing RPMI, 10% fetal bovine serum, 50 μM β-mercaptoethanol, and L-glutamine. The mean GM-CSF concentration was determined to be 9.5 pg/mL in spleen supernatant, 500.5 pg/mL in lung supernatant.

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  Cytometric bead array standard curve of MP00306-2

  Cytometric bead array standard curve of MP00306-2, Mouse GM-CSF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82861-9-PBS. Detection antibody: 82861-8-PBS. Standard: Eg0512. Range: 0.625-10 ng/mL.

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Product Information

82861-8-PBS targets Gm-csf as part of a matched antibody pair:

MP00306-2: 82861-9-PBS capture and 82861-8-PBS detection (validated in Cytometric bead array)

MP00306-3: 82861-10-PBS capture and 82861-8-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: colony stimulating factor 2 (granulocyte-macrophage)
• GenBank Accession Number: NM-009969
• Gene Symbol: GM-CSF
• Gene ID (NCBI): 12981
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.