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Glypican 3 Polyclonal antibody, PBS Only

Glypican 3 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25175-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Glypican 3 Polyclonal antibody (25175-1-AP)

    WB analysis of HepG2 using 25175-1-AP (same clone as 25175-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25175-1-AP (Glypican 3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25175-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Glypican 3 Polyclonal antibody (25175-1-AP)

    WB analysis of HEK-293 using 25175-1-AP (same clone as 25175-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25175-1-AP (Glypican 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25175-1-PBS in a different storage buffer formulation.

Product Information

25175-1-PBS targets Glypican 3 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glypican 3 fusion protein Ag10129
Full Name glypican 3
Calculated Molecular Weight 580 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC035972
Gene Symbol GPC3
Gene ID (NCBI) 2719
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus. They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Glypican 3 (GPC3) is highly expressed in many tissues during development and plays an important role in the regulation of embryonic growth (PMID: 22467855). Loss-of-function mutations of GPC3 result in the Simpson-Golabi-Behmel overgrowth syndrome (SGBS), and Gpc-3 null mice display developmental overgrowth (PMID: 8589713; 18477453). In hepatocellular carcinoma (HCC), the overexpression of glypican 3 has been demonstrated to be a reliable diagnostic indicator (PMID: 19212669; 22706665). The calculated molecular weight of native glypican 3 is 66 kDa, glycanated forms of glypican 3 have higher molecular weights than 66 kDa (PMID: 12851874; 16024626; 19574424).