Glycophorin A Monoclonal antibody, PBS Only (Detector)

Glycophorin A Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B12G10

Cat no : 66778-1-PBS

Synonyms

GpMiIII, GPErik, GPA, Glycophorin-A, CD235a

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Validation Data Gallery

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  WB analysis of human blood using 66778-1-Ig (same clone as 66778-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 66778-1-Ig (GYPA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50422-2

  Cytometric bead array standard curve of MP50422-2, GYPA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66778-2-PBS. Detection antibody: 66778-1-PBS. Standard:Ag8635. Range: 0.391-100 ng/mL.

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Product Information

66778-1-PBS targets Glycophorin A as part of a matched antibody pair:

MP50422-2: 66778-2-PBS capture and 66778-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Glycophorin A fusion protein Ag8635
• Full Name: glycophorin A (MNS blood group)
• Calculated Molecular Weight: 150 aa, 16 kDa
• Observed Molecular Weight: 36-38 kDa
• GenBank Accession Number: BC005319
• Gene Symbol: Glycophorin A
• Gene ID (NCBI): 2993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)