Glucocorticoid receptor Recombinant antibody
Glucocorticoid receptor Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
2F22
Cat no : 82619-4-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, A549 cells, U-251 cells, DU 145 cells, MCF-7 cells, L-929 cells, RAW 264.7 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:175-1:700 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
82619-4-RR targets Glucocorticoid receptor in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Glucocorticoid receptor fusion protein Ag21146 |
Full Name | nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) |
Calculated Molecular Weight | 86 kDa |
Observed Molecular Weight | 94-97 kDa |
GenBank Accession Number | BC015610 |
Gene Symbol | Glucocorticoid receptor |
Gene ID (NCBI) | 2908 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).
Protocols
Product Specific Protocols | |
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WB protocol for Glucocorticoid receptor antibody 82619-4-RR | Download protocol |
IHC protocol for Glucocorticoid receptor antibody 82619-4-RR | Download protocol |
IF protocol for Glucocorticoid receptor antibody 82619-4-RR | Download protocol |
IP protocol for Glucocorticoid receptor antibody 82619-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |