Giantin Polyclonal antibody

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  WB analysis using 22270-1-AP

  Various lysates were subjected to Tris-Acetate gel system followed by western blot with 22270-1-AP (Giantin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 22270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22270-1-AP (Giantin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 22270-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Giantin antibody (22270-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 22270-1-AP

  Immunofluorescent analysis of HepG2 cells using 22270-1-AP (Giantin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 22270-1-AP

  Immunofluorescent analysis of HeLa cells using 22270-1-AP (Giantin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Giantin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 22270-1-AP

Publications(7)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GOLGB1, 372 kDa Golgi complex-associated protein

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 4 publications below

Product Information

22270-1-AP targets Giantin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: golgin B1, golgi integral membrane protein
• Calculated Molecular Weight: 376 kDa
• Observed Molecular Weight: 376 kDa
• GenBank Accession Number: NM_004487
• Gene Symbol: Giantin
• Gene ID (NCBI): 2804
• RRID: AB_2879055
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14789
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Giantin is a conserved Golgi membrane protein containing a large cytoplasmic domain. It may function to form the intercisternal cross-bridges of the Golgi complex. Anti-Giantin has been widely used to label the Golgi apparatus.

Publications

Species	Application	Title
human	IF	iScience; Cytosolic Ca2+ Modulates Golgi Structure Through PKCα-Mediated GRASP55 Phosphorylation.; Authors - Stephen Ireland
human		Int J Nanomedicine; PMID: 28769563; Authors - Huiying Xue
human	IF	J Alzheimers Dis; High Fat Diet Mediates Amyloid-β Cleaving Enzyme 1 Phosphorylation and SUMOylation, Enhancing Cognitive Impairment in APP/PS1 Mice; Authors - Jian Bao
human	WB	Methods Mol Biol; Immunofluorescence Microscopy of the Mammalian Golgi Apparatus; Authors - Maryam Arab
monkey	IF	Seizure; Heterozygous RELN missense variants associated with genetic generalized epilepsy; Authors - Xiaoling Wu
human	IF	Nat Methods; StayGold variants for molecular fusion and membrane-targeting applications; Authors - Ryoko Ando

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (04-28-2023)

Detected a band around the expected size, however the signal was really weak.

• Applications: Western Blot