Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IHC detected in | human rectal cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells, HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22270-1-AP targets Giantin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | golgin B1, golgi integral membrane protein |
| Calculated Molecular Weight | 376 kDa |
| Observed Molecular Weight | 376 kDa |
| GenBank Accession Number | NM_004487 |
| Gene Symbol | Giantin |
| Gene ID (NCBI) | 2804 |
| RRID | AB_2879055 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14789 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Giantin is a conserved Golgi membrane protein containing a large cytoplasmic domain. It may function to form the intercisternal cross-bridges of the Golgi complex. Anti-Giantin has been widely used to label the Golgi apparatus.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Giantin antibody 22270-1-AP | Download protocol |
| IHC protocol for Giantin antibody 22270-1-AP | Download protocol |
| WB protocol for Giantin antibody 22270-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Giantin Polyclonal antibody (Cat# 22270-1-AP) has 11 citations. These appear in journals including Nature Methods, Science Advances, International Journal of Nanomedicine, iScience, Journal of Biological Chemistry, Journal of Alzheimer's Disease, Seizure, Biology Open, bioRxiv, and Methods in Molecular Biology. Research fields span Golgi apparatus structure and trafficking, membrane-targeting tools, cancer drug delivery, calcium signaling, neurodegeneration (Alzheimer's), epilepsy, and photoreceptor biology. Applications cited include IF, WB, and IHC across human, mouse, and monkey samples.
| Species | Application | Title |
|---|---|---|
Sci Adv The nuclear import receptor importin-7 targets HPV from the Golgi to the nucleus to promote infection. | ||
iScience Cytosolic Ca2+ Modulates Golgi Structure Through PKCα-Mediated GRASP55 Phosphorylation. | ||
J Biol Chem Molecular consequences of SCA5 mutations in the spectrin-repeat domains of β-III-spectrin | ||
J Alzheimers Dis High Fat Diet Mediates Amyloid-β Cleaving Enzyme 1 Phosphorylation and SUMOylation, Enhancing Cognitive Impairment in APP/PS1 Mice |
Reviews
What customers say
Users report mixed but generally positive experiences with this antibody. One reviewer, who tested it across Western Blot, IHC, and IF, praised its low background and overall performance. Another Western Blot user confirmed detection at the expected molecular weight but noted the signal was quite weak. Overall, the antibody appears specific and clean, though signal intensity may vary depending on the application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shazia (Verified Customer) (09-24-2025) | A very good giantin antibody with very less background
|
Boyan (Verified Customer) (04-28-2023) | Detected a band around the expected size, however the signal was really weak.
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