Giantin Polyclonal antibody

Giantin Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 22270-1-AP

Synonyms

Cell and organelle markers, GCP, GCP372, giantin, GOLGB1, Golgi marker, Golgin subfamily B member 1, GOLIM1, Macrogolgin



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

22270-1-AP targets Giantin in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name golgin B1, golgi integral membrane protein
Calculated molecular weight 376 kDa
Observed molecular weight 376 kDa
GenBank accession numberNM_004487
Gene symbol GOLGB1
Gene ID (NCBI) 2804
RRIDAB_2879055
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Giantin is a conserved Golgi membrane protein containing a large cytoplasmic domain. It may function to form the intercisternal cross-bridges of the Golgi complex. Anti-Giantin has been widely used to label the Golgi apparatus.

Protocols

Product Specific Protocols
WB protocol for Giantin antibody 22270-1-APDownload protocol
IF protocol for Giantin antibody 22270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

iScience

Cytosolic Ca2+ Modulates Golgi Structure Through PKCα-Mediated GRASP55 Phosphorylation.

Authors - Stephen Ireland
human

Int J Nanomedicine

PMID: 28769563

Authors - Huiying Xue
humanIF

J Alzheimers Dis

High Fat Diet Mediates Amyloid-β Cleaving Enzyme 1 Phosphorylation and SUMOylation, Enhancing Cognitive Impairment in APP/PS1 Mice

Authors - Jian Bao
humanWB

Methods Mol Biol

Immunofluorescence Microscopy of the Mammalian Golgi Apparatus

Authors - Maryam Arab
monkeyIF

Seizure

Heterozygous RELN missense variants associated with genetic generalized epilepsy

Authors - Xiaoling Wu
humanIF

Nat Methods

StayGold variants for molecular fusion and membrane-targeting applications

Authors - Ryoko Ando

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (04-28-2023)

Detected a band around the expected size, however the signal was really weak.

  • Applications: Western Blot