Tested Applications
Positive WB detected in | Jurkat cells, HEK-293 cells |
Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
84199-2-RR targets Geminin in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Geminin fusion protein Ag1076 Predict reactive species |
Full Name | geminin, DNA replication inhibitor |
Calculated Molecular Weight | 24 kDa |
Observed Molecular Weight | 28-29 kDa |
GenBank Accession Number | BC005185 |
Gene Symbol | GMNN |
Gene ID (NCBI) | 51053 |
RRID | AB_3671755 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | O75496 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in inhibiting DNA replication by preventing the incorporation of MCM complex into the pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to the apparent mass of 42.5 kDa and 66 kDa are present. The dimer of geminin(50 kDa) can also be detected. (PMID: 15313623)
Protocols
Product Specific Protocols | |
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WB protocol for Geminin antibody 84199-2-RR | Download protocol |
IF protocol for Geminin antibody 84199-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |