Gelsolin Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Gelsolin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgA

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C4A2

Cat no : 66280-1-PBS

Synonyms

Actin depolymerizing factor, ADF, AGEL, Brevin, Gelsolin, GSN

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Validation Data Gallery

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  WB analysis of U2OS using 66280-1-Ig (same clone as 66280-1-PBS)

  U2OS (WT and GSN KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 66280-1-Ig incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 66280-1-Ig (same clone as 66280-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 66280-1-Ig (same clone as 66280-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66280-1-Ig (same clone as 66280-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66280-1-Ig (same clone as 66280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66280-1-Ig (same clone as 66280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66280-1-Ig (same clone as 66280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66280-1-Ig (same clone as 66280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Product Information

66280-1-PBS targets Gelsolin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgA
• Class: Monoclonal
• Type: Antibody
• Immunogen: Gelsolin fusion protein Ag17916
• Full Name: gelsolin (amyloidosis, Finnish type)
• Calculated Molecular Weight: 782 aa, 86 kDa
• Observed Molecular Weight: 80-83 kDa
• GenBank Accession Number: BC026033
• Gene Symbol: Gelsolin
• Gene ID (NCBI): 2934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease.