Gamma Cystathionase Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Gamma Cystathionase Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C7F9

Cat no : 60234-1-PBS

Synonyms

CSE, CTH, Cystathionine gamma lyase, Gamma cystathionase

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Validation Data Gallery

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  WB analysis using 60234-1-Ig (same clone as 60234-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60234-1-Ig (Gamma Cystathionase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60234-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 cells using 60234-1-Ig (same clone as 60234-1-PBS)

  WB result of CTH antibody (60234-1-Ig, 1:500) with si-control and si-CTH transfected PC-3 cells. This data was developed using the same antibody clone with 60234-1-PBS in a different storage buffer formulation.

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  WB analysis using 60234-1-Ig (same clone as 60234-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60234-1-Ig (Gamma cystathionase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60234-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60234-1-Ig (same clone as 60234-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60234-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60234-1-Ig (same clone as 60234-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60234-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 60234-1-Ig (same clone as 60234-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60234-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60234-1-Ig (same clone as 60234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60234-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60234-1-Ig (same clone as 60234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60234-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60234-1-Ig (same clone as 60234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 60234-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60234-1-Ig (same clone as 60234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 60234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60234-1-Ig (same clone as 60234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:50. This data was developed using the same antibody clone with 60234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60234-1-Ig (same clone as 60234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:50. This data was developed using the same antibody clone with 60234-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60234-1-Ig (same clone as 60234-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60234-1-Ig(Gamma cystathionase antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60234-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60234-1-PBS targets Gamma Cystathionase in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Gamma Cystathionase fusion protein Ag2872
• Full Name: cystathionase (cystathionine gamma-lyase)
• Calculated Molecular Weight: 405 aa, 45 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC015807
• Gene Symbol: CTH
• Gene ID (NCBI): 1491
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CTH, also named as Gamma-cystathionase and CSE, belongs to the transsulfuration enzymes family. It catalyzes the last step in the transsulfuration pathway from methionine to cysteine. CTH converts two cysteine molecules to lanthionine and hydrogen sulfide. CTH can also accept homocysteine as substrate. It specificity depends on the levels of the endogenous substrates. CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. The CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion. Both male and female CTH-null mice showed hypercystathioninemia and hyperhomocysteinemia, but not hypermethioninemia. CSE has also been reported to be expressed in endothelial cells and contribute to endothelium-dependent vasorelaxation. It can be detected a minor 36 kDa band probably representing a degradative intermediate except the major 43 kDa band in vitamin B6-deficient rat liver(PMID:8660672). CTH also can be detected as ~70kD in rat liver (PMID: 18974309).