Gamma Cystathionase Monoclonal antibody

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• KD/KO Validated

Gamma Cystathionase Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

30

CloneNo.

2C7F9

Cat no : 60234-1-Ig

Synonyms

CSE, CTH, Cystathionine gamma lyase, Gamma cystathionase

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Validation Data Gallery

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  WB analysis using 60234-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60234-1-Ig (Gamma Cystathionase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of PC-3 cells using 60234-1-Ig

  WB result of CTH antibody (60234-1-Ig, 1:500) with si-control and si-CTH transfected PC-3 cells.

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  WB analysis using 60234-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60234-1-Ig (Gamma cystathionase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 60234-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60234-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 60234-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 60234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 60234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:400 (under 10x lens).

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  IHC staining of human liver cancer using 60234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human liver cancer using 60234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:50.

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  IHC staining of human liver cancer using 60234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:50.

• 

  IF Staining of HepG2 using 60234-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60234-1-Ig(Gamma cystathionase antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, HSC-T6 cells, PC-3 cells, HEK-293 cells, HeLa cells, A431 cells, NIH/3T3 cells, HepG2 cells, K-562 cells
• Positive IHC detected in: human lung cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 25 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Product Information

60234-1-Ig targets Gamma Cystathionase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Gamma Cystathionase fusion protein Ag2872
• Full Name: cystathionase (cystathionine gamma-lyase)
• Calculated Molecular Weight: 405 aa, 45 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC015807
• Gene Symbol: Gamma Cystathionase
• Gene ID (NCBI): 1491
• RRID: AB_2881358
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTH, also named as Gamma-cystathionase and CSE, belongs to the transsulfuration enzymes family. It catalyzes the last step in the transsulfuration pathway from methionine to cysteine. CTH converts two cysteine molecules to lanthionine and hydrogen sulfide. CTH can also accept homocysteine as substrate. It specificity depends on the levels of the endogenous substrates. CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. The CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion. Both male and female CTH-null mice showed hypercystathioninemia and hyperhomocysteinemia, but not hypermethioninemia. CSE has also been reported to be expressed in endothelial cells and contribute to endothelium-dependent vasorelaxation. It can be detected a minor 36 kDa band probably representing a degradative intermediate except the major 43 kDa band in vitamin B6-deficient rat liver(PMID:8660672). CTH also can be detected as ~70kD in rat liver (PMID: 18974309).

Publications

Species	Application	Title
		Sci Adv; Treatment with siRNAs is commonly associated with GPX4 up-regulation and target knockdown-independent sensitization to ferroptosis; Authors - Anne von Mässenhausen
human	WB	Sci Adv; Dexamethasone sensitizes to ferroptosis by glucocorticoid receptor-induced dipeptidase-1 expression and glutathione depletion.; Authors - Anne von Mässenhausen
human	WB	Autophagy; Vascular smooth muscle cell-derived hydrogen sulfide promotes atherosclerotic plaque stability via TFEB (transcription factor EB)-mediated autophagy.; Authors - Zhenzhen Chen
rat	WB	Biomed Pharmacother; Protective role of hydrogen sulfide against diabetic cardiomyopathy by inhibiting pyroptosis and myocardial fibrosis; Authors - Maojun Liu
rat	WB,IF	Oxid Med Cell Longev; Sodium Tanshinone IIA Sulfonate Attenuates Erectile Dysfunction in Rats with Hyperlipidemia.; Authors - Liren Zhong
human	WB	Int J Mol Sci; Fructose Intake Impairs the Synergistic Vasomotor Manifestation of Nitric Oxide and Hydrogen Sulfide in Rat Aorta.; Authors - Andrea Berenyiova