Filter: AllWBIHCIF

WB analysis of HT-29 using 66686-1-Ig (same clone as 66686-1-PBS)
HT-29 cells were subjected to SDS PAGE followed by western blot with 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.
WB analysis of human ileum using 66686-1-Ig (same clone as 66686-1-PBS)
human ileum tissue were subjected to SDS PAGE followed by western blot with 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.
IF Staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.
IF Staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

Galectin-4 Monoclonal antibody, PBS Only

Galectin-4 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 66686-1-PBS

Clone No.3C2G3

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Antigen NY CO 27, Gal 4, GAL4, Galectin-4, L 36 lactose binding protein, L36LBP, Lactose binding lectin 4, LGALS4

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66686-1-PBS targets Galectin-4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity	Human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	Galectin-4 fusion protein Ag4049 Predict reactive species
Full Name	lectin, galactoside-binding, soluble, 4
Calculated Molecular Weight	323 aa, 36 kDa
Observed Molecular Weight	36 kDa
GenBank Accession Number	BC034750
Gene Symbol	Galectin-4
Gene ID (NCBI)	3960
RRID	AB_2882040
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P56470
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.