Galectin-4 Monoclonal antibody, PBS Only

Galectin-4 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C2G3

Cat no : 66686-1-PBS

Synonyms

Antigen NY CO 27, Gal 4, GAL4, Galectin-4, L 36 lactose binding protein, L36LBP, Lactose binding lectin 4, LGALS4

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Validation Data Gallery

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  WB analysis of HT-29 using 66686-1-Ig (same clone as 66686-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

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  WB analysis of human ileum using 66686-1-Ig (same clone as 66686-1-PBS)

  human ileum tissue were subjected to SDS PAGE followed by western blot with 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

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Product Information

66686-1-PBS targets Galectin-4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Galectin-4 fusion protein Ag4049
• Full Name: lectin, galactoside-binding, soluble, 4
• Calculated Molecular Weight: 323 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC034750
• Gene Symbol: Galectin-4
• Gene ID (NCBI): 3960
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.