Galc Polyclonal antibody

Galc Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51049-2-AP

Synonyms

2310068B06Rik, A930008M05Rik, AW212969, AW413532, Gacy, galactosylceramidase, Galc, twi, twitcher

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Validation Data Gallery

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  WB analysis of A375 using 51049-2-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 51049-2-AP (Galc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 51049-2-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 51049-2-AP (Galc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 51049-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51049-2-AP (Galc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 51049-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51049-2-AP (Galc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 51049-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 51049-2-AP (Galc antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 51049-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 51049-2-AP (Galc antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: A375 cells, human brain tissue, human placenta tissue, mouse brain tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

51049-2-AP targets Galc in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Galc fusion protein Ag0684
• Full Name: galactosylceramidase
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC086671
• Gene Symbol: Galc
• Gene ID (NCBI): 14420
• RRID: AB_670391
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GALC, also named as Galactosylceramidase , belongs to the glycosyl hydrolase 59 family. It hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is an enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon. Normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits. (PMID:8634707 )