Glycophorin A/CD235a Recombinant antibody, PBS Only (Detector)

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  IHC staining of human placenta using 85182-3-RR (same clone as 85182-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85182-3-RR (GYPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85182-3-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 85182-3-RR (same clone as 85182-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85182-3-RR (GYPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85182-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01888-1

  Cytometric bead array standard curve of MP01888-1, Glycophorin A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85182-2-PBS. Detection antibody: 85182-3-PBS. Standard: Eg2336. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP01888-2

  Cytometric bead array standard curve of MP01888-2, Glycophorin A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85182-1-PBS. Detection antibody: 85182-3-PBS. Standard: Eg2336. Range: 0.313-40 ng/mL.

Glycophorin A/CD235a {ptg:Brand} Recombinant Antibody for IHC, Cytometric bead array, Indirect ELISA

Cat No. 85182-3-PBS

Clone No.242822H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Cytometric bead array, Indirect ELISA

Synonyms

Glycophorin A, GPErik, GPA, Glycophorin-A, CD235a

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Product Information

85182-3-PBS targets Glycophorin A/CD235a as part of a matched antibody pair:

MP01888-1: 85182-2-PBS capture and 85182-3-PBS detection (validated in Cytometric bead array)

MP01888-2: 85182-1-PBS capture and 85182-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: glycophorin A (MNS blood group)
• Calculated Molecular Weight: 150 aa, 16 kDa
• GenBank Accession Number: BC005319
• Gene Symbol: Glycophorin A
• Gene ID (NCBI): 2993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02724
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Glycophorin A (GYPA), also known as CD235a, is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). Glycophorin A represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)