GUCY2C Monoclonal antibody, PBS Only

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  WB analysis of Caco-2 using 67782-1-Ig (same clone as 67782-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67782-1-Ig (GUCY2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67782-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HaCaT using 67782-1-Ig (same clone as 67782-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 67782-1-Ig (GUCY2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67782-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67782-1-Ig (same clone as 67782-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67782-1-Ig (GUCY2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67782-1-PBS in a different storage buffer formulation.

GUCY2C Monoclonal Antibody for WB, Indirect ELISA

Cat No. 67782-1-PBS

Clone No.1F5F6

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

GC C, Guanylyl cyclase C, GUC2C, GUCY2C, hSTAR, Intestinal guanylate cyclase, STA receptor, STAR

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Product Information

67782-1-PBS targets GUCY2C in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GUCY2C fusion protein Ag19256
• Full Name: guanylate cyclase 2C (heat stable enterotoxin receptor)
• Calculated Molecular Weight: 1073 aa, 123 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC136544
• Gene Symbol: GUCY2C
• Gene ID (NCBI): 2984
• RRID: AB_2918546
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P25092
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

GUCY2C is also named as GUC2C, STAR(Heat-stable enterotoxin receptor), Guanylyl cyclase C and belongs to the adenylyl cyclase class-4/guanylyl cyclase family. It is a receptor for the E.coli heat-stable enterotoxin and considered to be the true apical membrane receptor for the endogenous ligands uroguanylin and guanylin. GUCY2C is not present in the endocrine pancreas, but is localized exclusively to the ductal system which fully corresponds to the GUCY2C activity detected in the exocrine ductal system, but particularly not in islets of Langerhans. GUCY2C with a molecular mass of 130 kDa is detected in pancreatic and intestinal extracts.(PMID:11431160). The full length protein is 123 kDa and has a signal peptide and nine glycosylation sites.