Uroguanylin Polyclonal antibody

Uroguanylin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 18113-1-AP

Synonyms

GUCA2B, UGN, Guanylate cyclase C-activating peptide 2, Guanylate cyclase activator 2B, GCAP II Uroguanylin

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Validation Data Gallery

• 

  WB analysis of mouse pancreas using 18113-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 18113-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 18113-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 18113-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 18113-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18113-1-AP (Uroguanylin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18113-1-AP

  Immunofluorescent analysis of HepG2 cells using 18113-1-AP (Uroguanylin antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, mouse colon tissue, mouse small intestine tissue, rat pancreas tissue
• Positive IHC detected in: human pancreas cancer tissue, human kidney tissue, human stomach tissue, human colon cancer tissue, mouse small intestine tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

18113-1-AP targets Uroguanylin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Uroguanylin fusion protein Ag12589
• Full Name: guanylate cyclase activator 2B (uroguanylin)
• Calculated Molecular Weight: 112 aa, 12 kDa
• Observed Molecular Weight: 12-13 kDa
• GenBank Accession Number: BC093779
• Gene Symbol: Uroguanylin
• Gene ID (NCBI): 2981
• RRID: AB_10858615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uroguanylin, also named as GUCA2B, is a candidate intestinal natriuretic hormone that controls the sodium and water balance between the intestine and the kidneys. It has been reported that the uroguanylin knockout mouse exhibits elevated blood pressure. Therefore, the GUCA2B gene is thought to be a susceptibility gene for essential hypertension (EH).

Publications

Species	Application	Title
mouse	IHC	Front Physiol; Decreased Colonic Guanylin/Uroguanylin Expression and Dried Stool Property in Mice With Social Defeat Stress.; Authors - Nobuhiko Ebisutani
human	WB	Hum Gene Ther; Gene Co-Expression and miRNA Regulation: A Path to Early Intervention in Colorectal Cancer; Authors - Jason C Huang

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