GTF3C6 Polyclonal antibody

GTF3C6 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26382-1-AP

Synonyms

TFIIIC35, NPD020, CDA020, C6orf51

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Validation Data Gallery

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  WB analysis of HeLa using 26382-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26382-1-AP (GTF3C6 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 26382-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26382-1-AP (GTF3C6 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 26382-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26382-1-AP (GTF3C6 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 26382-1-AP

  IP result of anti-GTF3C6 (IP:26382-1-AP, 4ug; Detection:26382-1-AP 1:2000) with HepG2 cells lysate 3600ug.

• 

  IHC staining of human urothelial carcinoma using 26382-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 26382-1-AP (GTF3C6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 26382-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26382-1-AP (GTF3C6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26382-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26382-1-AP (GTF3C6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human urothelial carcinoma tissue, human lung cancer tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26382-1-AP targets GTF3C6 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GTF3C6 fusion protein Ag23895
• Full Name: general transcription factor IIIC, polypeptide 6, alpha 35kDa
• Observed Molecular Weight: 35-38 kDa
• GenBank Accession Number: BC011593
• Gene Symbol: GTF3C6
• Gene ID (NCBI): 112495
• RRID: AB_2880497
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.