GTF3C1 Polyclonal antibody

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  WB analysis using 31679-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31679-1-AP (GTF3C1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

GTF3C1 Polyclonal Antibody for WB, ELISA

Cat No. 31679-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

DKFZp686A111, GTF3C1, TF3C alpha, TFIIIC, TFIIIC 220 kDa subunit, TFIIIC box B binding subunit, TFIIIC220, TFIIICalpha

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31679-1-AP targets GTF3C1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GTF3C1 fusion protein Ag36117
• Full Name: general transcription factor IIIC, polypeptide 1, alpha 220kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC137229
• Gene Symbol: GTF3C1
• Gene ID (NCBI): 2975
• RRID: AB_3670073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q12789
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription factor IIIC (TF3C or GTF3C), a component of RNA polymerase III, is a multisubunit complex that binds B box promoter elements. It is involved in the assembly of the transcription complex on the initiation tRNA and is required for the transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs (PMID: 8164661).