TFIIE Beta Polyclonal antibody

TFIIE Beta Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11596-1-AP

Synonyms

GTF2E2, TFIIE-beta, TFIIE B, TF2E2

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Validation Data Gallery

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  WB analysis of K-562 using 11596-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 11596-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11596-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of A375 using 11596-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using TFIIE Beta antibody (11596-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: K-562 cells, A375 cells, Jurkat cells
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

11596-1-AP targets TFIIE Beta in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFIIE Beta fusion protein Ag2136
• Full Name: general transcription factor IIE, polypeptide 2, beta 34kDa
• Calculated Molecular Weight: 291 aa, 33 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC030572
• Gene Symbol: GTF2E2
• Gene ID (NCBI): 2961
• RRID: AB_2232722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The general transcription factor IIE (TFIIE) is an essential component of the eukaryotic RNA polymerase II initiation complex, which plays pivotal roles in initiation of protein-coding gene transcription (PMID: 7829083). Transcription initiation factor IIE comprises two subunits, TFIIE-alpha and TFIIE-beta, two chains of which together form a tetramer. TFIIE-beta is encoded by GTF2E2 gene and contains TFIIE beta-DNA binding domain. It mainly functions to recruit TFIIH to the initiation complex and stimulate RNA polymerase II kinase activity (PMID: 10792464).

Publications

Species	Application	Title
mouse	WB	Nucleic Acids Res; Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation.; Authors - De Cegli Rossella R
human	WB	Cancer Gene Ther; GTF2E2 downregulated by miR-340-5p inhibits the malignant progression of glioblastoma; Authors - Xiaolong Qiao