GTF2B Polyclonal antibody

GTF2B Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16467-1-AP

Synonyms

GTF2B, S300 II, TF2B, TFIIB

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Validation Data Gallery

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  WB analysis of K-562 using 16467-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16467-1-AP (GTF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16467-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16467-1-AP (GTF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16467-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16467-1-AP (GTF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16467-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16467-1-AP (GTF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 16467-1-AP

  IP result of anti-GTF2B (IP:16467-1-AP, 4ug; Detection:16467-1-AP 1:300) with K-562 cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: K-562 cells, HepG2 cells, HeLa cells, MCF-7 cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16467-1-AP targets GTF2B in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GTF2B fusion protein Ag9547
• Full Name: general transcription factor IIB
• Calculated Molecular Weight: 316 aa, 35 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC020597
• Gene Symbol: GTF2B
• Gene ID (NCBI): 2959
• RRID: AB_2878263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	PLoS Biol; A novel TOX3-WDR5-ABCG2 signaling axis regulates the progression of colorectal cancer by accelerating stem-like traits and chemoresistance; Authors - Jiaojiao Hao
		Am J Cancer Res; PMID: 28123844; Authors - Jiaojiao Hao
human	WB	J Exp Clin Cancer Res; Melatonin synergizes BRAF-targeting agent vemurafenib in melanoma treatment by inhibiting iNOS/hTERT signaling and cancer-stem cell traits.; Authors - Jiaojiao Hao