GSTZ1 Polyclonal antibody

GSTZ1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 14889-1-AP

Synonyms

glutathione transferase zeta 1, GSTZ1, GSTZ1 1, MAAI, MAI, Maleylacetoacetate isomerase

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Validation Data Gallery

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  WB analysis using 14889-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14889-1-AP (GSTZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14889-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14889-1-AP (GSTZ1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 14889-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14889-1-AP (GSTZ1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14889-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14889-1-AP (GSTZ1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

14889-1-AP targets GSTZ1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSTZ1 fusion protein Ag6676
• Full Name: glutathione transferase zeta 1
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC001453
• Gene Symbol: GSTZ1
• Gene ID (NCBI): 2954
• RRID: AB_2116363
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glutathione transferase zeta 1(GSTZ1) catalyzes the biotransformation of a range of α-haloacids, including dichloroacetic acid (DCA), and the penultimate step in the tyrosine degradation pathway. GSTZ1 is preferentially expressed in hepatocytes and renal proximal tubule cells where phenylalanine and tyrosine are catabolized(PMID: 16550164). This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	IHC	Front Immunol; Identification and validation of a tyrosine metabolism-related prognostic prediction model and characterization of the tumor microenvironment infiltration in hepatocellular carcinoma; Authors - Yangying Zhou
mouse	WB	J Gerontol A Biol Sci Med Sci; PMID: 24285747; Authors - Rojanathammanee Lalida L
mouse	WB	Age (Dordr); PMID: 25001375; Authors - Lalida Rojanathammanee
rat	WB	BMC Complement Med Ther; Integrated proteomics and metabolomics analysis of lumbar in a rat model of osteoporosis treated with Gushukang capsules; Authors - Ruohui Lin
human	WB	ACS Chem Biol; Chemical Proteomics with Novel Fully Functionalized Fragments and Stringent Target Prioritization Identifies the Glutathione-Dependent Isomerase GSTZ1 as a Lung Cancer Target; Authors - Yi Liao