Tested Applications
Positive WB detected in | MCF-7 cells |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
55474-1-AP targets GSTM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide Predict reactive species |
Full Name | glutathione S-transferase mu 2 (muscle) |
Calculated Molecular Weight | 26 kDa |
Observed Molecular Weight | 26 kDa |
GenBank Accession Number | NM_000848 |
Gene Symbol | GSTM2 |
Gene ID (NCBI) | 2946 |
RRID | AB_3086453 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P28161 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glutathione S-Transferase Mu 2 (GSTM2) is a phase II metabolizing enzyme that conjugates glutathione to electrophilic compounds. GSTM2 is closely related to cancer progression, and there have been several publications regarding the function of GSTM2 in drug resistance (PMID: 36038661). Western blot analysis detected GSTM2 at an apparent molecular mass of 26 kDa.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for GSTM2 antibody 55474-1-AP | Download protocol |
IHC protocol for GSTM2 antibody 55474-1-AP | Download protocol |
IF protocol for GSTM2 antibody 55474-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |