Various lysates were subjected to SDS PAGE followed by western blot with 81527-1-RR (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 81527-1-RR (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 81527-1-RR
WB result of GSTK1 antibody (81527-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTK1 transfected HepG2 cells.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 81527-1-RR
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 81527-1-RR
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 81527-1-RR
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 81527-1-RR
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 81527-1-RR
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using GSTK1 antibody (81527-1-RR, Clone: 3J1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 81527-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSTK1 antibody (81527-1-RR, Clone: 3J1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSTK1 antibody (81527-1-RR, Clone: 3J1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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mouse testis tissue, mouse liver tissue, human pancreas cancer tissue, human lung cancer tissue, human liver cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse liver tissue
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity and hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).
Various lysates were subjected to SDS PAGE followed by western blot with 81527-1-RR (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 81527-1-RR
WB result of GSTK1 antibody (81527-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTK1 transfected HepG2 cells.
IHC Figures
IHC staining of mouse testis using 81527-1-RR
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 81527-1-RR
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 81527-1-RR
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 81527-1-RR
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 81527-1-RR
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 81527-1-RR
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of mouse liver using 81527-1-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using GSTK1 antibody (81527-1-RR, Clone: 3J1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 81527-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSTK1 antibody (81527-1-RR, Clone: 3J1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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