GSTA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GSTA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 14475-1-AP

Synonyms

Glutathione S transferase A1, EC:5.3.3.-, EC:2.5.1.18, EC:1.11.1.-, Androst-5-ene-3,17-dione isomerase

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Validation Data Gallery

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  WB analysis using 14475-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14475-1-AP (GSTA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14475-1-AP

  WB result of GSTA1 antibody (14475-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTA1 transfected HepG2 cells.

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  WB analysis of human kidney using 14475-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14475-1-AP (GSTA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14475-1-AP

  IP result of anti-GSTA1 (IP:14475-1-AP, 3ug; Detection:14475-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human lung cancer using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 14475-1-AP (GSTA1 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 14475-1-AP (GSTA1 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 14475-1-AP (GSTA1 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 14475-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GSTA1 antibody (14475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, human kidney tissue, HepG2 cells, mouse liver tissue, rat kidney tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human lung cancer tissue, human liver tissue, human colon cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 3 publications below

Product Information

14475-1-AP targets GSTA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSTA1 fusion protein Ag5849
• Full Name: glutathione S-transferase alpha 1
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC053578
• Gene Symbol: GSTA1
• Gene ID (NCBI): 2938
• RRID: AB_2232810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSTA1 encodes one of the GST alpha isozymes, belongs to GST family, which is mainly expressed in the liver. GST can improve the detoxification of organism by enzymatic and non-enzymatic methods, and has anti-mutagenic and anti-tumor effects. Studies have shown that overexpression of GSTA1 can promote the development of lung cancer cells, so the expression level of GSTA1 can be used to predict the degree of malignancy of lung cancer tissues. To some extent, GSTA1 is regarded as a potential lung cancer candidate targets.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions; Authors - Shengjie Fan
		Sci Adv; Treatment with siRNAs is commonly associated with GPX4 up-regulation and target knockdown-independent sensitization to ferroptosis; Authors - Anne von Mässenhausen
mouse	WB	Cell Death Dis; Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.; Authors - Cheng Wang
mouse	WB	J Gerontol A Biol Sci Med Sci; PMID: 24285747; Authors - Rojanathammanee Lalida L
mouse	WB	Age (Dordr); PMID: 25001375; Authors - Lalida Rojanathammanee
rat	WB	Food Chem Toxicol; l-Arginine induces antioxidant response to prevent oxidative stress via stimulation of glutathione synthesis and activation of Nrf2 pathway.; Authors - Mingcai Liang