CoraLite® Plus 488-conjugated GST tag Monoclonal antibody
GST tag Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2a
Reactivity
recombinant protein, schistosoma japonicum
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3B5F8
Cat no : CL488-66001
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Recombinant protein |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66001 targets GST tag in WB applications and shows reactivity with recombinant protein, schistosoma japonicum samples.
Tested Reactivity | recombinant protein, schistosoma japonicum |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Recombinant Protein |
Full Name | GST tag |
Calculated Molecular Weight | 26 kDa |
GenBank Accession Number | FN315687 |
Gene Symbol | |
Gene ID (NCBI) | |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins.
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 488 GST tag antibody CL488-66001 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |