CoraLite® Plus 488-conjugated GST tag Monoclonal antibody

GST tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein, schistosoma japonicum

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B5F8

Cat no : CL488-66001

Synonyms

GST Tag

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Validation Data Gallery

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  WB analysis using CL488-66001

  GST tagged recombinant protein lysates were subjected to SDS PAGE followed by western blot with CL488-66001 (GST tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66001 targets GST tag in WB applications and shows reactivity with recombinant protein, schistosoma japonicum samples.

• Tested Reactivity: recombinant protein, schistosoma japonicum
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: GST tag
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: FN315687
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins.