GSPT2 Polyclonal antibody

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  WB analysis using 12989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12989-1-AP (GSPT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12989-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12989-1-AP (GSPT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 12989-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12989-1-AP (GSPT2 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of MCF-7 using 12989-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GSPT2 antibody (12989-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

GSPT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12989-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GST2, G1 to S phase transition 2, Eukaryotic peptide chain release factor GTP-binding subunit ERF3B, eRF3b, EC:3.6.5.-

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Tested Applications

• Positive WB detected in: MCF-7 cells, NIH/3T3 cells, PC-3 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

12989-1-AP targets GSPT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSPT2 fusion protein Ag3641
• Full Name: G1 to S phase transition 2
• Calculated Molecular Weight: 628 aa, 69 kDa
• Observed Molecular Weight: 69-75 kDa
• GenBank Accession Number: BC036077
• Gene Symbol: GSPT2
• Gene ID (NCBI): 23708
• RRID: AB_2115511
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IYD1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSPT2, also named as Eukaryotic peptide chain release factor GTP-binding subunit ERF3B or G1 to S phase transition protein 2 homolog, is a 628 amino acid protein, which belongs to the GTP-binding elongation factor family. ERF3 subfamily. GSPT2 localizes in the cytoplasm and is highly expressed in IUCC stage II colorectal cancer (CRC). GSPT2 is involved in translation termination in response to the termination codons UAA, UAG and UGA and may play a role as a potent stimulator of the release factor activity of ETF1. It may play a role in cell cycle progression.

Publications

Species	Application	Title
human, mouse	WB	Mol Cell; Mammalian hyperplastic discs homolog EDD regulates miRNA-mediated gene silencing.; Authors - Hong Su
		Nat Commun; Noninvasive urinary protein signatures associated with colorectal cancer diagnosis and metastasis.; Authors - Yulin Sun
mouse	WB	RNA; Phosphorylation at intrinsically disordered regions of PAM2 motif-containing proteins modulates their interactions with PABPC1 and influences mRNA fate.; Authors - Huang Kai-Lieh KL
		Int J Mol Med; eRF3b-37 inhibits the TGF-β1-induced activation of hepatic stellate cells by regulating cell proliferation, G0/G1 arrest, apoptosis and migration.; Authors - Zhengrong Xu
human	IF	Cells; Relocalization of Translation Termination and Ribosome Recycling Factors to Stress Granules Coincides with Elevated Stop-Codon Readthrough and Reinitiation Rates upon Oxidative Stress; Authors - Desislava S Makeeva