GSNOR,ADH5 Monoclonal antibody

• Featured Product
• KD/KO Validated

GSNOR,ADH5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

5E6E2

Reviews (1)

Cat no : 66193-1-Ig

Synonyms

ADH 3, ADH5, ADHX, Alcohol dehydrogenase 5, Alcohol dehydrogenase class 3, FALDH, FDH, GSH FDH, GSNOR, GSNOR,ADH5

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Validation Data Gallery

• 

  WB analysis of HuH-7 using 66193-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66193-1-Ig

  WB result of GSNOR,ADH5 antibody (66193-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSNOR,ADH5 transfected HepG2 cells.

• 

  WB analysis of rat liver using 66193-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 66193-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66193-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66193-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66193-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66193-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 66193-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 66193-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 66193-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 66193-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 66193-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66193-1-Ig (GSNOR,ADH5 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HuH-7 cells, human testis tissue, HepG2 cells, fetal human brain tissue, pig liver tissue, rat brain tissue, rat liver tissue
• Positive IHC detected in: human pancreas cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Product Information

66193-1-Ig targets GSNOR,ADH5 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GSNOR,ADH5 fusion protein Ag9511
• Full Name: alcohol dehydrogenase 5 (class III), chi polypeptide
• Calculated Molecular Weight: 374 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC014665
• Gene Symbol: ADH5
• Gene ID (NCBI): 128
• RRID: AB_2881587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

Publications

Species	Application	Title
mouse	IF	Mol Psychiatry; Degradation of FA reduces Aβ neurotoxicity and Alzheimer-related phenotypes.; Authors - Xuechao Fei
mouse	IF,WB	Arterioscler Thromb Vasc Biol; Arginase-1 Deletion in Erythrocytes Promotes Vascular Calcification via Enhanced GSNOR (S-Nitrosoglutathione Reductase) Expression and NO Signaling in Smooth Muscle Cells; Authors - Rajinikanth Gogiraju
rat	IF	Front Physiol; Maintenance of Intestinal Homeostasis in Diarrhea-Predominant Irritable Bowel Syndrome by Electroacupuncture Through Submucosal Enteric Glial Cell-Derived S-Nitrosoglutathione; Authors - Yujun Hou
mouse	IF	Alzheimers Dement (N Y); New insight into Alzheimer's disease: Light reverses Aβ-obstructed interstitial fluid flow and ameliorates memory decline in APP/PS1 mice.; Authors - Xiangpei Yue
mouse	IF,CoIP	Eur J Pharmacol; N6022 attenuates cerebral ischemia/reperfusion injury-induced microglia ferroptosis by promoting Nrf2 nuclear translocation and inhibiting the GSNOR/GSTP1 axis; Authors - Wan-Li Duan
mouse	WB	FASEB J; Dysregulation of hepatic one-carbon metabolism in classical homocystinuria: Implications of redox-sensitive DHFR repression and tetrahydrofolate depletion for pathogenesis and treatment; Authors - Kenneth N Maclean

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (06-17-2021)

Good and strong antibody. However, it doesn't recognize the ADH5 from mouse origin. On the datasheet, it claims that this antibody works with mouse.

• Applications: Western Blot
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: HepG2, human liver and mouse liver

GSNOR,ADH5 Antibody Western Blot validation (1:8000 dilution) in HepG2, human liver and mouse liver (Cat no:66193-1-Ig)