GSNOR,ADH5 Polyclonal antibody

• Featured Product
• KD/KO Validated

GSNOR,ADH5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

41

Cat no : 11051-1-AP

Synonyms

ADH5, Alcohol dehydrogenase class-3, ADHX, ADH3, ADH 5

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Validation Data Gallery

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  WB analysis using 11051-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11051-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11051-1-AP

  IP result of anti-GSNOR,ADH5 (IP:11051-1-AP, 4ug; Detection:11051-1-AP 1:5000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of human prostate cancer using 11051-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11051-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 11051-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11051-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of K-562 using 11051-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human GSNOR,ADH5 (11051-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue, mouse kidney tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human prostate cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 32 publications below
• IHC: See 11 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Product Information

11051-1-AP targets GSNOR,ADH5 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSNOR,ADH5 fusion protein Ag1533
• Full Name: alcohol dehydrogenase 5 (class III), chi polypeptide
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC070491
• Gene Symbol: ADH5
• Gene ID (NCBI): 128
• RRID: AB_593422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

Publications

Species	Application	Title
human	WB	Blood; Analysis of disease model iPSCs derived from patients with a novel Fanconi anemia-like IBMFS ADH5/ALDH2 deficiency.; Authors - Anfeng Mu ; KO Validated
human	WB	Nat Commun; Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells; Authors - Akiko Tomita
human,mouse	WB,IHC	Mol Cell; Two Aldehyde Clearance Systems Are Essential to Prevent Lethal Formaldehyde Accumulation in Mice and Humans.; Authors - Felix A Dingler ; KO Validated
mouse	WB	Cancer Res; S-nitrosylation of the Mitochondrial Chaperone TRAP1 Sensitizes Hepatocellular Carcinoma Cells to Inhibitors of Succinate Dehydrogenase.; Authors - Salvatore Rizza ; KO Validated
mouse	WB	Antioxid Redox Signal; GSNOR deficiency enhances in situ skeletal muscle strength, fatigue resistance and RyR1 S-nitrosylation without impacting mitochondrial content and activity.; Authors - Younghye Moon ; KO Validated
mouse	WB	Redox Biol; GSNOR modulates hyperhomocysteinemia-induced T cell activation and atherosclerosis by switching Akt S-nitrosylation to phosphorylation.; Authors - Jing Li ; KD Validated

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