Gelsolin Polyclonal antibody

• Featured Product
• KD/KO Validated

Gelsolin Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 11644-2-AP

Synonyms

GSN, Actin-depolymerizing factor

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Validation Data Gallery

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  WB analysis using 11644-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11644-2-AP (Gelsolin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 11644-2-AP

  U2OS (WT and GSN KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 11644-2-AP incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of U2OS using 11644-2-AP

  U2OS lysates prepared and IP of GSN performed using 1.0 μg of 11644-2-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human colon cancer using 11644-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11644-2-AP (Gelsolin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 11644-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11644-2-AP (Gelsolin Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 11644-2-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11644-2-AP (Gelsolin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11644-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11644-2-AP (GSN antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: A549 cells, U2OS cells, HeLa cells
• Positive IP detected in: U2OS cells
• Positive IHC detected in: human colon cancer tissue, human colon tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

11644-2-AP targets Gelsolin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Gelsolin fusion protein Ag2239
• Full Name: gelsolin (amyloidosis, Finnish type)
• Calculated Molecular Weight: 782 aa, 86 kDa
• Observed Molecular Weight: 87-93 kDa
• GenBank Accession Number: BC026033
• Gene Symbol: Gelsolin
• Gene ID (NCBI): 2934
• RRID: AB_2295090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease. Plasma gelsolin can interact with actin and form a complex of 130 kDa (PMID: 6330060, 3030756).

Publications

Species	Application	Title
human	WB	Adv Healthc Mater; Modification of PLGA Scaffold by MSC-Derived Extracellular Matrix Combats Macrophage Inflammation to Initiate Bone Regeneration via TGF-β-Induced Protein.; Authors - Moyuan Deng
	WB	Genes Dev; Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein; Authors - Megan L Norris
human	WB,IF	Am J Cancer Res; 3'untranslated regions (3'UTR) of Gelsolin mRNA displays anticancer effects in non-small cell lung cancer (NSCLC) cells.; Authors - Xiaohui Liu
human	WB, IHC	Gene; Role of gelsolin in cell proliferation and invasion of human hepatocellular carcinoma cells.; Authors - Biao Deng
mouse	WB	Biomed Pharmacother; RACK1 promotes the proliferation, migration and invasion capacity of mouse hepatocellular carcinoma cell line in vitro probably by PI3K/Rac1 signaling pathway.; Authors - Wu Jun J
human	WB	Oncotarget; Correlation of five secretory proteins with the nasopharyngeal carcinoma metastasis and the clinical applications.; Authors - Ya Tao