GSK3A Polyclonal antibody

GSK3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 13419-1-AP

Synonyms

Serine/threonine-protein kinase GSK3A, GSK-3 alpha, GSK 3 alpha, Glycogen synthase kinase-3 alpha, EC:2.7.11.26

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Validation Data Gallery

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  WB analysis using 13419-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13419-1-AP (GSK3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 13419-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 13419-1-AP (GSK3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13419-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13419-1-AP (GSK3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 13419-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13419-1-AP (GSK3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13419-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13419-1-AP (GSK3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13419-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13419-1-AP (GSK3A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 13419-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13419-1-AP (GSK3A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 13419-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13419-1-AP (GSK3A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 13419-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13419-1-AP (GSK3A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 13419-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13419-1-AP (GSK3A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 13419-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13419-1-AP (GSK3A antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 13419-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSK3A antibody (13419-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HeLa cells, human plasma, mouse liver tissue, MCF-7 cells, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue, human lung cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

13419-1-AP targets GSK3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSK3A fusion protein Ag4168
• Full Name: glycogen synthase kinase 3 alpha
• Calculated Molecular Weight: 483 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC027984
• Gene Symbol: GSK3A
• Gene ID (NCBI): 2931
• RRID: AB_2247995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycogen synthase kinase-3 alpha (GSKA) is one of the two functional isoforms encoded by the GSK gene and 

acts as a tumor suppressor through its kinase function, targeting many different proteins.

What is the molecular weight of GSKA?

51 kDa. GSKA is a protein serine kinase composed of 483 amino acids and the catalytic domain shares a high 

degree of homology with GSKB, although they are encoded on separate genes. 

What is the function of GSKA?

Constitutively expressed across all tissues, GSKA has a large number of target proteins and is therefore involved 

in many different pathways (PMID: 19923896). It was first identified as a regulator of glycogen metabolism, as 

GSK3A phosphorylates the enzyme glycogen synthase, thereby inhibiting glycogenesis, the conversion of glucose 

into glycogen (PMID: 6249596). It has since been shown to interact with many other proteins and is one of the 

kinases with most substrates in the cell, with roles in cell cycle progression, proliferation, differentiation, and 

apoptosis (PMID: 17570479; PMID: 11563964). Typically, GSKA negatively regulates its downstream substrates, 

often tagging proteins for ubiquitination. It has important roles in the Wnt and PI3K signaling pathways.

What is the role of GSK3A in neurodegenerative disease?

GSK3A is known to interact with beta-amyloid and tau, proteins that aggregate in areas of the neuronal system 

as part of the pathology of neurodegenerative diseases such as Alzheimer's disease. It has been shown that 

GSK3A causes abnormal phosphorylation of the microtubule-binding protein tau in a way that reflects the 

pathology of Alzheimer's disease (PMID: 1334849; PMID: 1336152). GSKA has also been shown to process 

amyloid precursor protein to beta-amyloid, another protein that forms the inclusion bodies, or Lewy bodies, 

that are one of the hallmarks in Alzheimer's (PMID: 1276154). Another protein involved in the pathology of 

Alzheimer's is α-synuclein, which is known to be a substrate for GSK-3β phosphorylation (PMID: 24681994).

Publications

Species	Application	Title
mouse	WB	Neuropsychopharmacology; Upregulation of GSK3β Contributes to Brain Disorders in Elderly REGγ-knockout Mice.; Authors - Yiqing Lv
human	WB	Front Cell Dev Biol; A GSK3-SRF Axis Mediates Angiotensin II Induced Endothelin Transcription in Vascular Endothelial Cells.; Authors - Yuyu Yang
human	WB	iScience; MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.; Authors - Dong-Xue Ding
human	WB	J Cell Physiol; The Ubiquitin Ligase SCF(FBXW7α) Promotes ATA3 Degradation.; Authors - Nan Song
human	WB	Pathol Oncol Res; Knockdown of Sox2 Inhibits OS Cells Invasion and Migration via Modulating Wnt/β-Catenin Signaling Pathway.; Authors - Liang Tang
human	WB	World J Diabetes; Exploring the targets and molecular mechanism of glycyrrhetinic acid against diabetic nephropathy based on network pharmacology and molecular docking; Authors - Fan-Di Meng