GSDMD Monoclonal antibody, PBS Only

GSDMD Monoclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E6C7

Cat no : 66387-1-PBS

Synonyms

DF5L, DFNA5L, FKSG10, gasdermin D, GSDMD, GSDMDC1

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Validation Data Gallery

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  WB analysis using 66387-1-Ig (same clone as 66387-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66387-1-Ig (same clone as 66387-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 66387-1-Ig (same clone as 66387-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  WB analysis of human small intestine using 66387-1-Ig (same clone as 66387-1-PBS)

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  WB analysis of human colon using 66387-1-Ig (same clone as 66387-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 66387-1-Ig (same clone as 66387-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 66387-1-Ig (same clone as 66387-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 66387-1-Ig (same clone as 66387-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66387-1-Ig (same clone as 66387-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  WB analysis of EC109 using 66387-1-Ig (same clone as 66387-1-PBS)

  EC109 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66387-1-Ig (same clone as 66387-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

• 

  WB analysis of TF-1 using 66387-1-Ig (same clone as 66387-1-PBS)

  TF-1 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  IP experiment of THP-1 using 66387-1-Ig (same clone as 66387-1-PBS)

  IP result of anti-GSDMD (IP:66387-1-Ig, 4ug; Detection:66387-1-Ig 1:2000) with THP-1 cells lysate 1560 ug. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66387-1-Ig (same clone as 66387-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66387-1-Ig(GSDMD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66387-1-PBS targets GSDMD in WB, IP, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GSDMD fusion protein Ag16827
• Full Name: gasdermin D
• Calculated Molecular Weight: 484 aa, 53 kDa
• Observed Molecular Weight: 47-53 kDa
• GenBank Accession Number: BC008904
• Gene Symbol: GSDMD
• Gene ID (NCBI): 79792
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.