GSDMC Polyclonal antibody

• Featured Product
• KD/KO Validated

GSDMC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 27630-1-AP

Synonyms

gasdermin C, GSDMC, MLZE

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Validation Data Gallery

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  WB analysis of HeLa using 27630-1-AP

  WB result of GSDMC antibody (27630-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSDMC transfected HeLa cells.

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  WB analysis using 27630-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27630-1-AP (GSDMC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 27630-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27630-1-AP (GSDMC antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 27630-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27630-1-AP (GSDMC antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 4 publications below

Product Information

27630-1-AP targets GSDMC in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSDMC fusion protein Ag26365
• Full Name: gasdermin C
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: ~60 kDa
• GenBank Accession Number: NM_031415
• Gene Symbol: GSDMC
• Gene ID (NCBI): 56169
• RRID: AB_2880930
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Immunity; Disruption of the endopeptidase ADAM10-Notch signaling axis leads to skin dysbiosis and innate lymphoid cell-mediated hair follicle destruction.; Authors - Keiko Sakamoto
human	WB,IHC	Nat Cell Biol; PD-L1-mediated gasdermin C expression switches apoptosis to pyroptosis in cancer cells and facilitates tumour necrosis.; Authors - Junwei Hou ; KO Validated
human	WB,IHC	J Clin Invest; Gasdermin C sensitizes tumor cells to PARP inhibitor therapy in cancer models; Authors - Shuanglian Wang
human	IHC	Am J Cancer Res; Pyroptosis impacts the prognosis and treatment response in gastric cancer via immune system modulation.; Authors - Wanli Yang
mouse	WB	Front Oncol; siRNAs Targeting Mouse-Specific lncRNA AA388235 Induce Human Tumor Cell Pyroptosis/Apoptosis.; Authors - Yan-Ru Chen
mouse	WB	Exp Ther Med; Tetracaine hydrochloride induces macrophage pyroptosis through caspase‑1/11‑GSDMD signaling pathways; Authors - Ran Zhang