GSDMB Recombinant antibody

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  WB analysis using 86148-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86148-1-RR (GSDMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

GSDMB Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86148-1-RR

Clone No.250653H4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

gasdermin B, Gasdermin like protein, Gasdermin-B, Gasdermin-B, C-terminal, Gasdermin-B, N-terminal

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Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells, COLO 320 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86148-1-RR targets GSDMB in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: gasdermin B
• Calculated Molecular Weight: 411 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC025682
• Gene Symbol: GSDMB
• Gene ID (NCBI): 55876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TAX9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSDMB (Gasdermin B) is a member of the gasdermin family of proteins, which are involved in the regulation of pyroptosis, a form of programmed cell death. GSDMB plays a crucial role in immune responses, particularly against viral respiratory infections. It has also been implicated in various inflammatory and autoimmune diseases, as well as in cancer progression. GSDMB is known for its ability to induce pyroptosis by forming pores in cell membranes, leading to the release of inflammatory cytokines such as IL-1β and IL-18. This process is critical for combating infections and modulating immune responses. GSDMB can be activated by granzyme A (GZMA), which is released from natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), leading to inflammatory cell death and influencing the tumor immune microenvironment. GSDMB also interacts with other signaling pathways, such as the ERK pathway, to regulate cell proliferation and death. (PMID: 40452932; 38066523; 36163066)