GSDMB Monoclonal antibody, PBS Only

GSDMB Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F12C4

Cat no : 67527-1-PBS

Synonyms

gasdermin B, Gasdermin like protein, GSDMB, GSDML, PP4052, PRO2521

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 67527-1-Ig (same clone as 67527-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67527-1-Ig (GSDMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67527-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 67527-1-Ig (same clone as 67527-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67527-1-Ig (GSDMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67527-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 67527-1-Ig (same clone as 67527-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67527-1-Ig (GSDMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67527-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67527-1-Ig (same clone as 67527-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSDMB antibody (67527-1-Ig, Clone: 2F12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67527-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67527-1-PBS targets GSDMB in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GSDMB fusion protein Ag28332
• Full Name: gasdermin B
• Calculated Molecular Weight: 411 aa, 47 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC025682
• Gene Symbol: GSDMB
• Gene ID (NCBI): 55876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.