GSDMA Polyclonal antibody

GSDMA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30354-1-AP

Synonyms

FKSG9, FLJ39120, Gasdermin 1, gasdermin A, GSDM, GSDM1, GSDMA

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Validation Data Gallery

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  WB analysis using 30354-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30354-1-AP (GSDMA antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: COLO 320 cells, Daudi cells, mouse stomach tissue, mouse skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30354-1-AP targets GSDMA in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSDMA fusion protein Ag31284
• Full Name: gasdermin A
• Calculated Molecular Weight: 445 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC109197
• Gene Symbol: GSDMA
• Gene ID (NCBI): 284110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gasdermins, a family of five pore-forming proteins (GSDMA-GSDME) in humans expressed predominantly in the skin, mucosa and immune sentinel cells, are key executioners of inflammatory cell death (pyroptosis), which recruits immune cells to infection sites and promotes protective immunity. GSDMA was predicted to enable phosphatidylinositol-4,5-bisphosphate binding activity, phosphatidylinositol-4-phosphate binding activity, and phosphatidylserine binding activity. Involved in apoptotic process.