GRP94 Recombinant antibody, PBS Only

GRP94 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6G15

Cat no : 82805-2-PBS

Synonyms

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP

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Validation Data Gallery

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  WB analysis using 82805-2-RR (same clone as 82805-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82805-2-RR (GRP94 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82805-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 82805-2-RR (same clone as 82805-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82805-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 82805-2-RR (same clone as 82805-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82805-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 82805-2-RR (same clone as 82805-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82805-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 82805-2-RR (same clone as 82805-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82805-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human normal colon using 82805-2-RR (same clone as 82805-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82805-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82805-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82805-2-RR against Human GRP94 were performed. The affinity constant is 101 nM.

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Product Information

82805-2-PBS targets GRP94 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GRP94 fusion protein Ag6344
• Full Name: heat shock protein 90kDa beta (Grp94), member 1
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC066656
• Gene Symbol: GRP94
• Gene ID (NCBI): 7184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.