GRP78/BIP Monoclonal antibody, PBS Only

GRP78/BIP Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6F7

Cat no : 66574-1-PBS

Synonyms

BIP, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSPA5, MIF2

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Validation Data Gallery

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  WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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  WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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  IP experiment of SGC-7901 using 66574-1-Ig (same clone as 66574-1-PBS)

  IP result of anti-GRP78/BIP (IP:66574-1-Ig, 5ug; Detection:66574-1-Ig 1:3000) with SGC-7901 cells lysate 2200 ug. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66574-1-Ig (same clone as 66574-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66574-1-Ig (same clone as 66574-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66574-1-Ig (same clone as 66574-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66574-1-Ig (same clone as 66574-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66574-1-Ig (same clone as 66574-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66574-1-PBS targets GRP78/BIP in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GRP78/BIP fusion protein Ag2188
• Full Name: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 70-78 kDa
• GenBank Accession Number: BC020235
• Gene Symbol: GRP78/BIP
• Gene ID (NCBI): 3309
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.