GRP78/BIP Monoclonal antibody

• Featured Product
• KD/KO Validated

GRP78/BIP Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse and More (4)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

71

CloneNo.

1D6F7

Reviews (1)

Cat no : 66574-1-Ig

Synonyms

BIP, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSPA5, MIF2

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Validation Data Gallery

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  WB analysis using 66574-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 66574-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66574-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66574-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SGC-7901 using 66574-1-Ig

  IP result of anti-GRP78/BIP (IP:66574-1-Ig, 5ug; Detection:66574-1-Ig 1:3000) with SGC-7901 cells lysate 2200 ug.

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  IHC staining of human liver cancer using 66574-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66574-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66574-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human colon cancer using 66574-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of human breast cancer using 66574-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66574-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66574-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, A549 cells, MCF-7 cells, Jurkat cells, HSCT6 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, SP2/0 cell, 4T1 cells
• Positive IP detected in: SGC-7901 cells
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 63 publications below
• IHC: See 10 publications below
• IF: See 15 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below

Product Information

66574-1-Ig targets GRP78/BIP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GRP78/BIP fusion protein Ag2188
• Full Name: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 70-78 kDa
• GenBank Accession Number: BC020235
• Gene Symbol: GRP78/BIP
• Gene ID (NCBI): 3309
• RRID: AB_2881934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.

Publications

Species	Application	Title
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
human	WB	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu
human,mouse	WB,IHC,IF	Int J Biol Macromol; p20BAP31 promotes cell apoptosis via interaction with GRP78 and activating the PERK pathway in colorectal cancer; Authors - Xiaohan Jiang
human,mouse	WB	Phytomedicine; Asperosaponin VI protects alcohol-induced hepatic steatosis and injury via regulating lipid metabolism and ER stress; Authors - Linlin Wei
mouse	WB	J Control Release; Glutathione-responsive copper-disulfiram nanoparticles for enhanced tumor chemotherapy; Authors - Meixu Chen
human	WB	Mol Oncol; The synthetic oleanane triterpenoid CDDO-2P-Im binds GRP78/BiP to induce unfolded protein response-mediated apoptosis in myeloma; Authors - George Luo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-19-2024)

Worked well at 1:2000, 5ug lysate

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: MSCs

GRP78/BIP Antibody Western Blot validation (1:2000 dilution) in MSCs (Cat no:66574-1-Ig)