GRP Polyclonal antibody

GRP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 28482-1-AP

Synonyms

BN, gastrin releasing peptide, GRP, GRP 10, preproGRP, proGRP

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Validation Data Gallery

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  WB analysis using 28482-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 28482-1-AP (GRP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 28482-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28482-1-AP (GRP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28482-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28482-1-AP (GRP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 28482-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28482-1-AP (GRP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 28482-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28482-1-AP (GRP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

28482-1-AP targets GRP in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRP fusion protein Ag29357
• Full Name: gastrin-releasing peptide
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16-18 kDa
• GenBank Accession Number: BC004488
• Gene Symbol: GRP
• Gene ID (NCBI): 2922
• RRID: AB_2881152
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Ann N Y Acad Sci; The von Economo neurons in the frontoinsular and anterior cingulate cortex.; Authors - John M Allman
human	IHC	Front Immunol; A novel necroptosis-related gene index for predicting prognosis and a cold tumor immune microenvironment in stomach adenocarcinoma; Authors - Muhammad Khan