GRLF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GRLF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 26789-1-AP

Synonyms

ARHGAP35, GRF 1, GRF1, GRLF1, KIAA1722, P190 A, P190A, p190RhoGAP, Rho GAP p190A

---

Validation Data Gallery

• 

  WB analysis of A549 using 26789-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26789-1-AP (GRLF1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 26789-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26789-1-AP (GRLF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 26789-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26789-1-AP (GRLF1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 26789-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26789-1-AP (GRLF1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 26789-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26789-1-AP (GRLF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 26789-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26789-1-AP (GRLF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: A549 cells, HeLa cells
• Positive IHC detected in: human colon tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

26789-1-AP targets GRLF1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRLF1 fusion protein Ag25353
• Full Name: glucocorticoid receptor DNA binding factor 1
• Observed Molecular Weight: 172 kDa
• GenBank Accession Number: BC150257
• Gene Symbol: GRLF1
• Gene ID (NCBI): 2909
• RRID: AB_2880636
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRLF1 is also named as ARHGAP35, p190ARHOGAP, KIAA1722, P190A and GRF1. GRLF1 is a kind of Rho GTPase-activating protein, and it binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PMID:19673492). This binding is inhibited by phosphorylation by PRKCA (PMID:19673492). GRLF1 is Involved in cell differentiation as well as cell adhesion and migration. And it plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis.

Publications

Species	Application	Title
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu ; KD Validated
human	WB,IF	Oncogene; Ubiquitin ligase TRIM65 promotes colorectal cancer metastasis by targeting ARHGAP35 for protein degradation.; Authors - Daici Chen
human	WB	Front Cell Dev Biol; Feedback-Driven Mechanisms Between Phosphorylated Caveolin-1 and Contractile Actin Assemblies Instruct Persistent Cell Migration.; Authors - Xuemeng Shi
human	WB	Cell Death Discov; The m6A demethylases FTO and ALKBH5 aggravate the malignant progression of nasopharyngeal carcinoma by coregulating ARHGAP35; Authors - Zhiyuan Yang