NMDAR2B/GRIN2B Monoclonal antibody

NMDAR2B/GRIN2B Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1C5E12

Reviews (1)

Cat no : 66565-1-Ig

Synonyms

GluN2B, GRIN2B, hNR3, NMDAR2B, NMDAR2B/GRIN2B, NR2B, NR3

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Validation Data Gallery

• 

  WB analysis of mouse brain using 66565-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66565-1-Ig (NMDAR2B/GRIN2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66565-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66565-1-Ig (NMDAR2B/GRIN2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66565-1-Ig targets NMDAR2B/GRIN2B in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NMDAR2B/GRIN2B fusion protein Ag16718
• Full Name: glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• Calculated Molecular Weight: 1484 aa, 166 kDa
• Observed Molecular Weight: 166 kDa
• GenBank Accession Number: BC113620
• Gene Symbol: GRIN2B
• Gene ID (NCBI): 2904
• RRID: AB_2881926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRIN2B (also known as GluN2B or NMDAR2B) is a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. NMDA receptors are widely expressed in the central nervous system and play a major role in excitatory synaptic transmission and plasticity (PMID: 23223336). NMDA receptors large multi-subunit complexes arranged into heteromeric assemblies composed of four homologous subunits within a repertoire of over 10 different subunits: eight GluN1 isoforms, four GluN2 subunits (A-D) and two GluN3 subunits (A and B) (PMID: 21395862). Naturally occurring mutations within GRIN2B gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia.

Publications

Species	Application	Title
mouse	WB	Philos Trans R Soc Lond B Biol Sci; Cage effects on synaptic plasticity and its modulation in a mouse model of fragile X syndrome; Authors - Rasa Volianskis

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lisa (Verified Customer) (10-04-2021)

The antibody signal is not very strong and specific on hiPSCs derived neurons: it is detectable with an exposure of about 90 seconds, but sometimes gives aspecific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSCs-derived neurons