GPRIN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPRIN1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13771-1-AP

Synonyms

GPRIN1, GRIN1, KIAA1893

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Validation Data Gallery

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  WB analysis of mouse brain using 13771-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13771-1-AP (GPRIN1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13771-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13771-1-AP (GPRIN1 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13771-1-AP

  IP result of anti-GPRIN1 (IP:13771-1-AP, 3ug; Detection:13771-1-AP 1:500) with mouse brain tissue lysate 3600ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13771-1-AP targets GPRIN1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPRIN1 fusion protein Ag4754
• Full Name: G protein regulated inducer of neurite outgrowth 1
• Calculated Molecular Weight: 1008 aa, 102 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC052950
• Gene Symbol: GPRIN1
• Gene ID (NCBI): 114787
• RRID: AB_2114013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPRIN1 (G protein-regulated inducer of neurite outgrowth 1), also named GRIN1, may be involved in neurite outgrowth. Western blotting analysis has confirmed its expression in mouse neuroblastoma and rat pheochromocytoma cells. And both GPRIN1 and GNAO1 are membrane-bound proteins that are enriched in the growth cones of neurites (PMID:10480904). It has 2 isoforms produced by alternative splicing. This antibody detects GRIN1 at bands around 110 kDa.

Publications

Species	Application	Title
mouse	WB,IHC,IF	Sci Signal; Dynamic interactions of the 5-HT6 receptor with protein partners control dendritic tree morphogenesis.; Authors - Camille N Pujol ; KD Validated
mouse	WB	J Biol Chem; DHHC5 interacts with PDZ domain 3 of post-synaptic density-95 (PSD-95) protein and plays a role in learning and memory.; Authors - Li Yi Y
human	WB	Exp Ther Med; Chronic ethanol exposure induces SK-N-SH cell apoptosis by increasing N-methyl-D-aspartic acid receptor expression and intracellular calcium.; Authors - Hongbo Wang

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