GRB10 Polyclonal antibody

GRB10 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16622-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis of HepG2 using 16622-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16622-1-AP (GRB10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16622-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16622-1-AP (GRB10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 16622-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16622-1-AP (GRB10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16622-1-AP targets GRB10 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRB10 fusion protein Ag9921
• Full Name: growth factor receptor-bound protein 10
• Calculated Molecular Weight: 536aa,61 kDa; 594aa,67 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC024285
• Gene Symbol: GRB10
• Gene ID (NCBI): 2887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Growth factor receptor-bound protein 10 (GRB10) is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including the ins (INSR) and ins-like growth factor (IGF1R) receptors. It may play a role in mediating ins-stimulated ubiquitination of INSR, leading to proteasomal degradation.