GPX8 Recombinant antibody

• 

  WB analysis using 85120-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85120-1-RR (GPX8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

GPX8 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 85120-1-RR

Clone No.242769C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

GSHPx8, GSHPx 8, GPx-8, GPx 8, EC:1.11.1.9

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, U-87 MG cells, U2OS cells, NIH/3T3 cells, rat uterus tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85120-1-RR targets GPX8 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GPX8 fusion protein Ag9930
• Full Name: glutathione peroxidase 8 (putative)
• Calculated Molecular Weight: 209 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC029424
• Gene Symbol: GPX8
• Gene ID (NCBI): 493869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TED1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPX8(Probable glutathione peroxidase 8) is also named as GSHPx-8 and belongs to the glutathione peroxidase family. Glutathione peroxidase (GPx) reduces hydroperoxides, including hydrogen peroxides, in the presence of reduced glutathione as a means of protecting organisms from oxidative damage. Several GPx isozymes have been identified in animal cells and these have been classified into different groups according to their cellular location and substrate specificity. In humans, eight types of GPx have been identified from GPx1 to GPx8. The full length GPX8 has 209 amino acids and the molecular weight is 24 kDa.