Filter: AllWBIHCIF/ICC

WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.
WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.
WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 67763-1-Ig (same clone as 67763-1-PBS)
WB result of GPX4 antibody (67763-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 transfected HEK-293 cells. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.
WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.
WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67763-1-Ig (same clone as 67763-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67763-1-Ig (same clone as 67763-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.
IHC staining of rat testis using 67763-1-Ig (same clone as 67763-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide not using 67763-1-Ig (GPX4 antibody) as negtive control. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.
IHC staining of rat testis using 67763-1-Ig (same clone as 67763-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.
IHC staining of rat testis using 67763-1-Ig (same clone as 67763-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide not using 67763-1-Ig (GPX4 antibody) as negtive control. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 67763-1-Ig (same clone as 67763-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GPX4 antibody (67763-1-Ig, Clone: 3F5G5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

GPX4 Monoclonal antibody, PBS Only

GPX4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67763-1-PBS

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, dog

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

GSHPx 4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67763-1-PBS targets GPX4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, dog samples.

Tested Reactivity	human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, dog
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	GPX4 fusion protein Ag30650 Predict reactive species
Full Name	glutathione peroxidase 4 (phospholipid hydroperoxidase)
Observed Molecular Weight	20-23 kDa
GenBank Accession Number	BC021567
Gene Symbol	GPX4
Gene ID (NCBI)	2879
RRID	AB_2909469
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P36969
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.