GPX3 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPX3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 13947-1-AP

Synonyms

GPXP, GPx-3, GPx P, GPx 3, Glutathione peroxidase 3

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Validation Data Gallery

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  WB analysis of mouse kidney using 13947-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13947-1-AP (GPX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 13947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat eye using 13947-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat eye using 13947-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 13947-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GPX3 antibody (13947-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IHC detected in: rat eye tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below

Product Information

13947-1-AP targets GPX3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPX3 fusion protein Ag5054
• Full Name: glutathione peroxidase 3 (plasma)
• Calculated Molecular Weight: 226 aa, 26 kDa
• Observed Molecular Weight: 20-27 kDa
• GenBank Accession Number: BC050378
• Gene Symbol: GPX3
• Gene ID (NCBI): 2878
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPX3 (glutathione peroxidase 3) is also named as Plasma Glutathione Peroxidase. GPX3 can protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. GPX3 is secreted into plasma and like GPX1, is an 100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. It can be detected a band of 20 kDa which is considered as the chain A of GPX3 (selenocysteine to glycine mutant).

Publications

Species	Application	Title
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
mouse	WB	Theranostics; TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.; Authors - Kailiang Zhou
rat	WB	Oxid Med Cell Longev; The Secretome Deregulations in a Rat Model of Endotoxemic Shock.; Authors - A Blangy-Letheule
mouse	WB	Bioorg Chem; Two resveratrol analogs, pinosylvin and 4,4'-dihydroxystilbene, improve oligoasthenospermia in a mouse model by attenuating oxidative stress via the Nrf2-ARE pathway.; Authors - Cheng-Niu Wang
mouse	WB	Sci Rep; Selenocysteine insertion sequence binding protein 2 (Sbp2) in the sex-specific regulation of selenoprotein gene expression in mouse pancreatic islets.; Authors - B Chellan
chicken	WB	J Cell Physiol; Effect of Gpx3 gene silencing by siRNA on apoptosis and autophagy in chicken cardiomyocytes.; Authors - Yafan Gong ; KD Validated