GPR37/Pael-R Polyclonal antibody, PBS Only

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  WB analysis of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14820-1-AP (same clone as 14820-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GPR37/Pael-R Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 14820-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

GPR37, Pael-R, G-protein coupled receptor 37, ETBR-LP-1, ETBR LP 1

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Product Information

14820-1-PBS targets GPR37/Pael-R in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR37/Pael-R fusion protein Ag6591
• Full Name: G protein-coupled receptor 37 (endothelin receptor type B-like)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC040007
• Gene Symbol: GPR37
• Gene ID (NCBI): 2861
• RRID: AB_2232549
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15354
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

The orphan G protein-coupled receptor GPR37, also known as PAELR (parkin-associated endothelin receptor-like receptor) or ETBR-LP-1 (endothelin B receptor-like protein 1), is a 613 aa, multi-pass membrane protein predominantly expressed in the brain. It is a substrate of parkin (PARK2). When overexpressed in cells, GPR37 tends to become insoluble, unfolded, and ubiquitinated in vivo. Accumulation of the unfolded protein may lead to dopaminergic neuronal death in juvenile Parkinson disease (JPD). This antibody recognizes endogenous GPR37, which migrates as an approximately 50-55 kDa band in SDS-PAGE (PMID:17519329).