GPR177 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPR177 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 17950-1-AP

Synonyms

C1orf139, EVI, G protein coupled receptor 177, GPR177, MRP, Protein wntless homolog, Sprinter, WLS, Wntless

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Validation Data Gallery

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  WB analysis of HEK-293 using 17950-1-AP

  WB result of GPR177 antibody (17950-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPR177 transfected HEK-293 cells.

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  WB analysis using 17950-1-AP

  Various cell lysates were subjected to SDS PAGE followed by western blot with 17950-1-AP (GPR177 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 17950-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17950-1-AP (GPR177 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17950-1-AP

  IP result of anti-GPR177 (IP:17950-1-AP, 3ug; Detection:17950-1-AP 1:500) with mouse liver tissue lysate 12500ug.

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  IHC staining of human ovary using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse liver using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17950-1-AP (GPR177 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse liver using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17950-1-AP (GPR177 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: U-87 MG cells, mouse brain tissue, HEK-293 cells, rat brain tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human ovary tissue, mouse liver tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 4 publications below

Product Information

17950-1-AP targets GPR177 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR177 fusion protein Ag12428
• Full Name: G protein-coupled receptor 177
• Calculated Molecular Weight: 541 aa, 62 kDa
• Observed Molecular Weight: 46-65 kDa
• GenBank Accession Number: BC110826
• Gene Symbol: GPR177
• Gene ID (NCBI): 79971
• RRID: AB_2034928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736).

Publications

Species	Application	Title
human	WB	Cell Res; Comprehensive profiling reveals mechanisms of SOX2-mediated cell fate specification in human ESCs and NPCs.; Authors - Chenlin Zhou
rat	WB	Front Physiol; A Longitudinal Study of T2 Mapping Combined With Diffusion Tensor Imaging to Quantitatively Evaluate Tissue Repair of Rat Skeletal Muscle After Frostbite.; Authors - Yue Gao
human	WB,IHC	Onco Targets Ther; Wntless (Wls): A Prognostic Index for Progression and Patient Survival of Breast Cancer.; Authors - Dandan Zheng
mouse	IHC	Reprod Sci; WNT5A Enhances LH-Mediated Expression of HAS2 in Granulosa Cells.; Authors - Qun Niu
mouse	WB	Int Ophthalmol; The effect of Wnt/β-catenin pathway on the scleral remolding in the mouse during form deprivation.; Authors - Shuyu Hu
goat	IHC	Cells; Integrative Analysis of Methylome and Transcriptome Reveals the Regulatory Mechanisms of Hair Follicle Morphogenesis in Cashmere Goat.; Authors - Shanhe Wang