GPR124 Polyclonal antibody

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  IHC staining of mouse small intestine using 20050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20050-1-AP (GPR124 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 20050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20050-1-AP (GPR124 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR124 Polyclonal Antibody for IHC, ELISA

Cat No. 20050-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

Tumor endothelial marker 5, TEM5, KIAA1531, G-protein coupled receptor 124, G protein coupled receptor 124

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Tested Applications

• Positive IHC detected in: mouse small intestine tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20050-1-AP targets GPR124 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: G protein-coupled receptor 124
• Calculated Molecular Weight: 143 kDa
• GenBank Accession Number: NM_032777
• Gene Symbol: GPR124
• Gene ID (NCBI): 25960
• RRID: AB_3669320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96PE1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR124, also named KIAA1531 and TEM5, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. GPR124 is an Orphan receptor. GPR124 is a Tumor endothelial marker (TEM) that is significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. GPR124 is a member of the adhesion family of G protein-coupled receptors and is localized on the surface of endothelial cells. GPR124 is a seven-pass transmembrane receptor, unlike TEM1, TEM7, and TEM8 which span the membrane once. Proteolytically processed soluble GPR124 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans. The antibody recognizes near the C-term of GPR124. The antibody can't recognize the isoform3 of GPR124.