GPR112 Polyclonal antibody

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  IHC staining of mouse colon using 31781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31781-1-AP (GPR112 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 31781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31781-1-AP (GPR112 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 31781-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using GPR112 antibody (31781-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

GPR112 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 31781-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

RP1 299I16, PGR17, G protein coupled receptor 112

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Tested Applications

• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31781-1-AP targets GPR112 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR112 fusion protein Ag36114
• Full Name: G protein-coupled receptor 112
• Calculated Molecular Weight: 333kDa
• GenBank Accession Number: NM_153834
• Gene Symbol: GPR112
• Gene ID (NCBI): 139378
• RRID: AB_3670112
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8IZF6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR112 is a member of the adhesion G protein-coupled receptor (aGPCR) family. These receptors are characterized by a complex architecture and large size, mainly attributed to their N terminus, which contains a variety of domains involved in cell-cell and cell-matrix interactions. The function of GPR112 and other aGPCRs is closely related to key aspects of the development, architecture, and function of the nervous system. They are involved in neuronal migration, axon guidance, dendritogenesis, and synaptogenesis. Additionally, aGPCRs, including GPR112, have been implicated in the development of myelinating glial cells, which are crucial for proper neuronal signal propagation.